Tutorial: Restriction Enzyme Digests

This tutorial will show you how to work with restriction enzyme digest.

  1. Open the GI Notebook you saved in Tutorial 13: Customizing and Saving Analysis Setup Suites, and select the restriction enzyme analysis output object. Make it bigger by dragging the lower right corner handle down and to the right.
  2. Double-click it to make it the target and then select Object Edit Display Parameters…. This will bring up Figure 35. Set the display to show only unique cutters by checking the two check boxes at the bottom of the window and then placing a 1 in each text box (as in Figure 35). Press OK. This will display only those enzyme which cut exactly once.
    Figure 35: Restriction Enzyme Display Parameters

  3. Click on the AatI enzyme name to select it. Hold down the shift key and click on the name AvrII to select all the enzymes that are unique cutters. Now, while the digests are still highlighted, use the Format menu to change the color to green and the style to bold.
  4. Choose Object Edit Display Parameters again and now enter a 100 into the “no more than” text field. This specifies that you want to see all enzymes that cut at least once but not more than 100 times. Press the OK button. Note that the color/style of the unique cutters is preserved, but all other digests are in their original color of red.
  5. Choose Object View as Table to see the digests shown in tabular form. Note that when displayed as a table, you can change the font attributes. The filtering (allowable number of cuts) of which enzymes to display applies to both the table and graphic views. To switch back to the graphical view, choose Object View as Graphic.

This concludes this tutorial. You may quit or continue on to the next tutorial. If you choose to continue, close all open windows now.

This entry was posted in gene inspector tutorials and tagged , , , . Bookmark the permalink. Post a comment or leave a trackback: Trackback URL.

Post a Comment

Your email is never published nor shared. Required fields are marked *


You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <s> <strike> <strong>