Tutorial: Shotgun Cloning
There is often a need to fragment a piece of DNA with a restriction enzyme and then place each of those fragments into a vector. This process, called shotgun cloning, can be conducted automatically for you by GCK3.
Tutorial: PCR Analysis
We will use the PCR analysis code to amplify a promoter region of a Drosophila hsp70 gene.
Tutorial: Translating Across Introns
When importing genomic sequences, there is typically a need to deal with exons and introns in order to determine protein sequences. This can be done easily in GCK3. This tutorial illustrates how the process works.
Tutorial: Searching and Retrieving Sequence Files from GenBank
The following steps will demonstrate how to search and retrieve sequences from GenBank and have them automatically imported into GCK.
Tutorial: Importing GenBank Sequencing Files Using Deluxe Importing
Through the Deluxe Import feature, Gene Construction Kit contains comprehensive features to allow GenBank searching and file retrieval over the Internet with importing directly into GCK. All GenBank features are converted one-to-one to GCK features using a conversion scheme you can customize. The following tutorial illustrates the basics of these features. Images alternate between Macintosh [...]
Tutorial: Importing and Exporting Sequences and Other Information
One often obtains sequences in other formats and would like to work with them in GCK. This can be done by importing the sequences into GCK. Most other formats really are just plain text files with the sequence information organized in a specific format. Perhaps the most common of these formats is GenBank or GCG [...]
Tutorial: Working with Generic Constructs
There are times, especially when you are just beginning to work with a piece of DNA, when you might know the restriction map of a cloned fragment of DNA but you might not yet know the sequence. Such generic constructs can be created in GCK and the segments manipulated just like any piece of DNA [...]
Tutorial: Making Illustrations
In the previous tutorials you worked with one file type at a time – either a Construct window or a Gel window. However, when you are designing a cloning strategy it is often desirable to include many different constructs and gels in the same document along with descriptive text. This is the function of the [...]
Tutorial: Running Gels and Orientation Analysis
Often in a cloning project there is a need to assay the success of each cloning step. Typically, this is done by gel electrophoresis. This tutorial will teach you how to run gels in GCK.
Tutorial: Database Searching