32hentry p1 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-electrophoresis-gel-simulation tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-restriction-enzyme-digest-pattern tag-tutorials comments-open pings-open y2009 m12 d23 h03 slug-tutorial-running-gels-and-orientation-analysis

Tutorial: Running Gels and Orientation Analysis

Often in a cloning project there is a need to assay the success of each cloning step. Typically, this is done by gel electrophoresis. This tutorial will teach you how to run gels in GCK.
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30hentry p2 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-construct-management tag-file-management tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-manage-plasmid-collection tag-search-for-dna tag-search-plasmid-comments tag-tutorials comments-open pings-open y2009 m12 d23 h03 alt slug-tutorial-finding-comments-and-file-searching

Tutorial: Finding Comments and File Searching

You have seen in previous tutorials how you can enter comments in association with site markers, segments of DNA, chronographic generations, and regions of interest. Given that you may have many generations in a construct, it might sometimes be difficult not only to remember where in a construct you have stored information, but in which […]
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28hentry p3 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-chronography tag-construct-history tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-tutorials comments-open pings-open y2009 m12 d23 h03 slug-tutorial-chronography-tracking-cloning-history

Tutorial: Chronography – Tracking Cloning History

One of the most useful features of GCK is called chronography . This feature allows you to keep track of construct history as well as to store different views of the same sequence in a single file. It is important to realize that although you will be seeing different graphics on screen, there is only […]
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24hentry p4 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-silent-mutation tag-tutorials comments-open pings-open y2009 m12 d21 h05 alt slug-tutorial-cloning-a-dna-segment-and-silent-mutations

Tutorial: Cloning a DNA Segment and Silent Mutations

Our goal in this tutorial will be to take the construct we have been working with in previous tutorials and use it to receive a new segment of DNA. In doing this you will learn about copying and pasting segments of DNA and how to keep track of the history of your construct.
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19hentry p5 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-format-dna-sequence tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-graphic-plasmid-map tag-tutorials comments-open pings-open y2009 m12 d21 h03 slug-tutorial-modifying-the-construct-appearance

Tutorial: Modifying the Construct Appearance

Gene Construction Kit provides many different ways for you to annotate your construct’s appearance and mark important features.
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17hentry p6 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-format-dna-sequence tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-tutorials comments-open pings-open y2009 m12 d21 h03 alt slug-tutorial-viewing-the-construct-as-a-sequence

Tutorial: Viewing the Construct as a Sequence

Construct files can be viewed either graphically or as a sequence.
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13hentry p7 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-identify-dna-coding-region tag-open-reading-frame tag-orf tag-translate-dna tag-tutorials comments-open pings-open y2009 m12 d21 h03 slug-tutorial-marking-open-reading-frames

Tutorial: Marking Open Reading Frames

One analytical tool included in GCK is to automatically find open reading frames.
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10hentry p8 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-mark-restriction-enzyme-sites tag-restriction-enzyme-analysis tag-restriction-enzyme-digest tag-restriction-enzyme-map tag-tutorials comments-open pings-open y2009 m12 d10 h08 alt slug-tutorial-marking-sites-in-gene-construction-kit

Tutorial: Marking Sites in Gene Construction Kit

Marking sites is a common task faced by our users that is made simple and intuitive by the Gene Construction Kit. Follow these simple steps to learn how you can tag sites with our software.
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3hentry p9 post publish author-textco-biosoftware category-gene-construction-kit-tutorial tag-dna-cloning-software tag-dna-constructs tag-gene-construction-kit tag-gene-construction-kit-tutorials tag-plasmid-mapping-software tag-plasmids tag-restriction-enzyme-analysis-software tag-simulate-dna-cloning tag-tutorials comments-open pings-open y2009 m12 d10 h08 slug-tutorial-working-with-contructs-in-gene-construction-kit

Tutorial: Working with Contructs in Gene Construction Kit

Perhaps the most important window in GCK is the Construct Window . This is the place where you will do the majority of your cloning manipulations and can precisely define how your construct will look. In this tutorial you will learn how to modify the appearance of constructs. Tutorial 2: Marking Sites shows how to […]
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